Excretion of Glucose-Containing Oligosaccharides in Urines of Orthopedic Patients

Abstract

Urinary neutral oligosaccharides of various connective tissue diseases were studied by gel-filtration through Sephadex G-10 after treatments with cetylpyridinium chloride (CPC), Dowex 50 (H⁺ form) and Dowex 1 (Cl^- form), in succession. Increased excretion of urinary glucose-containing oligosaccharides, specifically glucosylgalactose was observed in most of the patients with chondrosarcoma, rheumatoid arthritis, Werner's syndrome, Rothmund Thomson syndrome and Morquio's disease. However, urinary excretion of neutral oligosaccharides in the patients with osteosarcoma and other tumorous conditions, and some systemic disorders in the connective tissues, examined in the present study, showed almost normal values. It is indicated, therefore, that the activity of glucosidase is insufficient for the glucose-containing oligosaccharides produced from the ground substance(s) in the former type connective tissue diseases.