The Tohoku Journal of Experimental Medicine
Online ISSN : 1349-3329
Print ISSN : 0040-8727
ISSN-L : 0040-8727
Effects of In Vitro Treatment with 4-Hydroperoxycyclophosphamide and Hyperthermia on Leukemic Progenitor Cells
NAOKO MINEGISHIMASAYOSHI MINEGISHISHIGERU TSUCHIYATASUKE KONNO
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1989 Volume 159 Issue 2 Pages 101-111

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Abstract

MINEGISHI, N., MINEGISHI, M., TSUCHIYA, S. and KONNO, T. Effects of In Vitro Treatment with 4-Hydroperoxycyclophosphamide and Hyperthermia on Leukemic Progenitor Cells. Tohoku J. Exp. Med., 1989, 159 (2), 101-111-A key point of autologous bone marrow transplantation for leukemic patients is how to remove leukemic cells from their own bone marrow grafts. In this study a leukemic progenitor cell assay was used to evaluate the antileukemic efficacy of marrow-purging protocols that employed hyperthermia or 4-hydroperoxycyclophosphamide (4HC) against leukemic blasts obtained from patients. After the treatment of 2×107 nucleated bone marrow cells/ml with 100μg/ml of 4HC in the presence of 7% suspension of packed autologous erythrocytes, leukemic colonies were eradicated in 10 of 13 cases and reduced to less than 0.3% as compared with the colony count in untreated cultures in two cases. More than 10% of leukemic progenitor cells survived after hyperthermia treatment (42°C 60 min) in 7 of 9 cases. It is suggested that treatment of leukemic cells and 7% autologous erythrocytes with 100μg/ml of 4HC is effective to eliminate leukemic progenitor cells. Treatment with hyperthermia may not be effective enough to eliminate leukemic progenitor cells from autologous bone marrow.

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