Studies on the Route and Time of Injection of Donor Cells for the Induction of Acquired Tolerance in the Skin Homotransplantation in Mice

Abstract

1) Skin homotransplantation was performed in the fourth to fifth week after birth of uniform dd-strain mice which had been injected subcutaneously with the whole blood or spleen cell suspension obtained from the prospective donors (C 57 BL/6 Ms mice) 8 to 24 hours after birth. It was successful in considerably high percentage. Homografts survived for a long period of time and the growing of black hair inhered in the donor was also observed in many cases.
2) Subcutaneous injection of the cells from homologous origin was effective even after 16 to 18 days after birth.
3) If the recipients were X-irradiated shortly after birth, the tolerance range was prolonged evidently against controls without irradiation.