Studies on X-Agent

VI. Effect of X-Agent on Red Blood Cells

Abstract

Various phenomena, apparently concerned with the X-agent, which were previously found with the bacteria and the protein solutions have been demonstrated with human red blood cells.
It is believed that red cells, when mixed with an adequate hemolytic agent, such as hypotonic salt solution, will liberate a quantity of hemoglobin corresponding to the degree of change induced in the cells by the X-agent, so that the effect of the X-agent can be estimated by the measurement of the liberated hemoglobin amount.
Since the hemoglobin liberation or the hemolytis is irreversible, the effect of the X-agent could be estimated in this manner without fearing of reversibility of the change induced by the agent.