The Tohoku Journal of Experimental Medicine
Online ISSN : 1349-3329
Print ISSN : 0040-8727
ISSN-L : 0040-8727
Assay of Plasma Renin Substrate in Man
Masaru Maebashi, Kaoru Yoshinaga, Mitsuyasu Aida, Makio Okuyama, Atsushi Oikawa
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1965 Volume 85 Issue 4 Pages 299-304

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Abstract

Human renin was extracted according to Helmer's method. In order to purify renin in a form free from angiotensinase, two procedures were used; one was fractionation with ammonium sulfate and the other was pH treatment, acidification to pH 2.9 and alkalinization to pH 9.8. Incubating plasmas with this renin, renin substrate (angiotensinogen) was estimated in 20 normotensive subjects. No quantitative correlation was found between renin and its substrate.

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