氷結晶生成が魚肉の細胞外マトリクスに及ぼす 影響についての組織学的研究

―免疫染色を用いた氷結晶痕と細胞外マトリクスの同時観察―

抄録

The extracellular matrix (ECM) of frozen tissue has been histologically investigated by applying the cryocoating method improved by Fink (1986) as well as the immunohistochemical staining. Sections of frozen tuna tissue for light microscopy was obtained by the cryocoating method that consisted of coating the surface of the precut tissues by a coating media and drying it until a coating film was formed. Additionally, an immunohistochemical staining was attempted to stain the ECM in the section of tuna tissue prepared by the cryocoating method. The target molecular of the immunohistochemical staining was laminin that is one of the major proteins included in the ECM. As results, it was found that the cryocoating method could prepare the sections with minimal alteration of the ice crystal shapes in frozen tuna as well as Kawamoto method. By using the immunohistochemical staining combined with the cryocoating method, it was revealed that the ice crystals caused damages not only on the cytoplasm but also on the ECM. Further, image analysis told us that the area ratio of the ECM is decreased considerably from 4.8% to 1.0% during freezing, while the cytoplasm is less decreased from 95.2% to 50.0%.