Abstract
Osteoblast patterning on silicone rubber was investigated by using mesenchymal stem cells (MSC) and surface modification of the carbon negative-ion implantation. The ions were implanted at 3×1015 ions/cm2 and 10 keV through a micro-pattern mask. This mask consisted of the slit apertures of 50 μm in width and 150 μm in spacing. After 3 days cultures of osteoblast-like cell and MSC in the cell-growth media, only MSC adhesion could be patterned on the implanted region. After long-term culture of the patterned MSC in the osteogenic induction medium, the cells changed their cell shape from the elongated spread to the rounded spread and the osteoblast markers of type-I collagen and osteocalcin could be detected. The detections after 25 days culture indicated the osteoblast differentiation. As a result, the differentiation of MSC on the hydrophilic treatment patterning of carbon negative-ion implantation could be used for patterning osteoblast.
