抄録
Glucose-6-Phosphate dehydrogenase activities were measured in erythrocyte of healthy individuals and diabetic patients, and in order to elucidate relation between erythrocyte G6PD activity and diabetes, effects of insulin on erythrocytes and their hemolysates of diabetes in vitro were examined. Following results were obtained:
1) The erythrocyte G6PD activity of an untreated diabetic patient was significantly lower than that of a healthy individual. But after treatment with insulin or sulfonylurea, the value of activity of this enzyme was restored to that of a healthy individual.
2) When erythrocyte of untreated diabetic patients, added with 0.2 unit of insulin, was incubated for 6 hours in vitro, significant rise of G6PD activity was observed.
3) When G6P was added to erythrocytes or hemolysates, a significant rise in the activity of this enzyme was noticed, but when insulin was furthermore added to either of them, no rise in the activity was noticed.
Considering results above mentioned, the mechanism of the rise in the activity of this enzyme was discussed.
