インスリンレセプター研究用の豚肝細胞膜の分離法
1977 年 20 巻 4 号 p. 488-491
詳細
1977 年 20 巻 4 号 p. 488-491
Plasma membranes of pig liver were isolated by means of an aqueous two-phase polymer system and sucrose gradient. The plasma membranes were demonstrated to have high purity when measured by 5'-nucleotidase activity.
To our knowledge, this is the first demonstration of radioreceptor assay for insulin using pig liver plasma membranes. The technical performance of the assay is easy and rapid, and the assay system has a detection limit of 15μU/m/ of porcine insulin.
A radioreceptor assay for insulin should provide a useful tool for evaluation of biological activity of circulating human insulin and its derivatives.
