糖尿病
Online ISSN : 1881-588X
Print ISSN : 0021-437X
ISSN-L : 0021-437X
脂肪細胞のphosphatidylinositol 3-kinaseに対するインスリンの影響
黒田 義彦中山 秀隆桑島 悟青木 伸三橋 智子伊東 智浩中川 昌一
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1992 年 35 巻 1 号 p. 37-40

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It has been shown that a significant number of growth factors transduce intracellular signals via the same pathway which is induced by oncogene products. Recently, it has been reported that insulin receptor associates with PI3-kinase and activates production of the D-3 phosphoinositides.
We studied the influence of insulin on PI 3-kinase activity in the isolated rat adipocyte.
Insulin treatment of adipocytes caused a remarkable increase in the PI kinase activity in immunoprecipitates induced by anti-phosphotyrosine antibody. PI kinase activity was half-maximal at 1.15×10-8M of insulin. Enzyme activity in the immunoprecipitates from insulin-stimulated adipocytes was capable of phosphorylating phosphatidylinositol, phosphatidyl-inositol4-phosphate and phosphatidyl-inositol 4, 5-bisphosphate. A deacylated product of phosphorylated phosphatidyl-inositol corresponding to phosphatidylinositol 3-phosphate, was eluted on anion exchange HPLC at 34 min. This demonstrates that insulin regulates PI 3-kinase activity not only in cultured cell lines, but also in isolated rat adipocytes.

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