EFFECTS OF VARIOUS CHEMICALS ON THE DEVELOPMENT OF MOUSE URINARY BLADDER TUMORS INDUCED BY N-BUTYL-N-(4-HYDROXYBUTYL) NITROSAMINE

Abstract

Effects of various chemicals on the development of urinary bladder tumors in mouse treated with N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) were examined, Male C3H/He mice received 0.05% BBN in drinking water for 8 weeks, and were subsequently treated with basal diet containing trisodium nitrilotriacetate monohydrate (Na₃NTA·H₂O), nitrilotriacetic acid (H₃NTA), sodium saccharin, and DL-tryptophan for 12 weeks. DL-tryptophan tended to induce a more significant increase in neoplastic and dysplastic lesions of the urinary bladder as compared to Na₃NTA·H₂O, H₃NTA and sodium saccharin. These data showed that DL-tryptophan was more effective than Na₃NTA·H₂O, H₃NTA and sodium saccharin with regard to promoting potential on the development of urinary bladder tumors in mouse treated with BBN.