The karyotypes of three lotic snails, Semisulcospira kurodai, S. habei and S. fluvialis were surveyed at 3 localities in Japan, including the type locality of respective species. The observed diploid chromosome number was as follows: Semisulcospira kurodai 36, S. habei 18, S. fluvialis 26. Chromosome numbers and karyotypes were different from one another. Their karyotypes were different from three lotic species reported previously.