Abstract
The effect of 1-amino-2-ribitylamino-4,5-dimethylbenzene (diamine derivative) on the riboflavin formation by various intestinal bacteria was observed for the purpose of studying the mechanism of riboflavin biosynthesis. (1) Diamine derivative was effective in the formation of riboflavn. The highest formation was found when, glycine and urea were added to diamine derivative. (2) The riboflavin formation by diamine derivative was successful when washed cells of Escherichia coli communior were used, but not when the homogenate and cell free extract of the bacteria were used. (3) The ratio of FMN and free riboflavin to total riboflavin was generallly high in the addition of diamine derivative.
