Abstract
The authors found that Takadiastase contained an esterase which was capable to hydrolyse dibenzoylthiamine with a comparatively high potency. So an attempt was made to establish a method for determination of the total thiamine in foodstuffs, enriched with dibenzoylthiamine, by pretreating with Takadiastase. Some fundametal investigations were made and the following results were obtained : (1) The hydrolyzed product was recognized as thiamine by the paper partition chromatography (n-butanol・acetic acid・water=4 : 1 : 5). (2) At the optimum conditions (pH 5.5,37-50℃, Takadiastase concentration 0.5%), dibenzoylthiamine was quantitatively hydrolyzed in ten hours and could be determined by the thiochrome method with a nearly 100 per cent recovery.
