VITAMINS
Online ISSN : 2424-080X
Print ISSN : 0006-386X
CHEMICAL STUDIES ON FLAVINADENINEDINUCLEOTIDE : (XVI) PURIFICATION AND CONCENTRATION OF FAD SOLUTION WITH ACTIVE CARBON
Tokio KOBAYASHI
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1960 Volume 20 Pages 386-389

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Abstract
Elimination of salts from FAD solution eluted from anion exchange resins was tried by the use of active carbon. The procedure was simultaneously used for concentrating a dilute solution of FAD. After a dilute solution of FAD (25μg/cc) containing 0.1N NaCl was subjected to a thin layer of active carbon, the salt was washed away from the layer with water, and then the layer was washed again with acetone. The second washing with acetone made the following elution of FAD easy. FAD solution of 40 fold concentration was obtained with the recovery of 89% by the procedure mentioned above using a mixture of acetone and p-cresol saturated water (3 : 7) as the solvent for elution.
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© 1960 THE VITAMIN SOCIETY OF JAPAN

この記事はクリエイティブ・コモンズ [表示 - 非営利 - 改変禁止 4.0 国際]ライセンスの下に提供されています。
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
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