Candida albicans 4888によるビタミンB_6生合成にかんする研究 : (IV)コハク酸-2,3^<14>CのビタミンB_6へのとりこみ
1966 年 33 巻 4 号 p. 357-360
詳細
1966 年 33 巻 4 号 p. 357-360
The incorporation of radioactive carbon from succinic acid-2,3^<14>C into vitamin B_6 produced by the growing cell of Candida albicans 4888 was investigated. Vitamin B_6-^<14>C was isolated with Amberlite CG-50 ionexchange chromatography, thin layer chromatography, and repeated paper chromatography. Radioactive and bioautographic peaks of produced vitamin B_6 was in agreement with pyridoxal. On the other hand, it was recognized by radioautography that fumaric, malic, and citric acids were produced in the culture medium, especially remarkable accumulation of fumaric acid was observed.
