Abstract
A temperature-sensitive thiazoleless mutant, E. coli KG 801,was isolated from E. coli K12 after treatment with N-methyl-N'-nitro-N-nitrosoguanidine. The mutant grew in minimal medium with glucose or glycerol at 30℃, whereas it failed to grow with glucose at 37℃ and required thiazole for its growth. Among various amino acids tested, glycine was found to be able to replace thiazole for the growth of the mutant in the presence of glucose at 37℃. Glyoxylic acid and trimethyleneglycol were somwhat effective on the growth. On the other hand the growth of the mutant was inhibited when glucose, fructose or glyceraldehyde was added to a culture growing in the presence of glycerol at 37℃.
