Abstract
The enzymatic reduction of disulfide type thiamine derivatives to thiamine was investigated. In the homogenates of rat tissues except blood, there exist an enzyme which catalyzes the thiol-disulfide exchange reaction between reduced glutathione and disulfide type thiamine derivatives and the enzyme was named GSH-thiamine derives transhydrogenase. The enzyme was partially purified from rat liver and its substrate specificity was studied. This enzyme appeared to be different from some reported enzymes which catalyzed thiol-disulfide exchange reaction and seemed to play an important role for the reduction of disulfide type thiamine derives to thiamine in the body.
