Abstract
It was clarified in the previous report that pyridoxal and pyridoxal phosphate were uptaken by the ascites-sarcoma cells and these uptake processes depended upon temperature, properties of incubation medium, and energy supply. In order to clarify the relationship between the uptake of these vitamins and intracellular metabolism, intracellular lactate, phosphate, and ATP were determined under the conditions of uptake of these vitamins. Intracellular lactate and ATP corresponded almost in parallel with the uptaken amount of the vitamins. Pyridoxal phosphokinase activity was found in the soluble fraction of homogenate of the cells but not in the membrane fraction. The enzyme activity was inhibited moderately by 4-deoxypyridoxine.
