2018 年 61 巻 3 号 p. 143-149
We have experimentally investigated the effects of gas-liquid interfacial plasmas generated by jet type and in-solution pulse discharge type plasma devices on drug molecule transfer through the cell membrane toward developing minimally-invasive and highly-efficient drug transfer system. After the plasma generation above adherent living cells (direct plasma irradiation), the drug-simulated molecule (YOYO-1) transfer into the cells was clearly observed. In addition, it was observed that the YOYO-1 transfer was also enhanced after indirect plasma irradiation, and efficacy of the solution treated by the in-solution type plasma device was significantly higher than that in the jet type one and was suppressed by the addition of OH scavenger. OH radical measurement showed that the duration of OH radical generation using the in-solution type plasma device was longer than that in the jet type one. These results imply that OH radical as short-lived reactive species is one of the factors which enhance the YOYO-1 transfer.
