YAKUGAKU ZASSHI
Online ISSN : 1347-5231
Print ISSN : 0031-6903
ISSN-L : 0031-6903
一般論文
ミセル動電クロマトグラフィーによる生薬分析(2) ―ビャクジュツ中の Atractylenolide III の分析―
桑原 由佳里中村 考志橋本 晶夫西  博行
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2003 年 123 巻 6 号 p. 453-461

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We have investigated a method to analyze the marker substance of natural medicines by employing capillary electrophoretic techniques such as micellar electrokinetic chromatography (MEKC). We previously reported a simple MEKC assay method for calycosin in Astragali Radix and its fluid extract. In this investigation, we analyzed the marker substance atractylenolide III in Atractylodis Rhizoma. Atractylenolide III was clearly separated from the other components in A. Rhizoma and its dried extract by simple solvent extraction with hexane and subsequent MEKC analysis with sodium dodecyl sulfate (SDS) within 10min. Validation of the analytical procedures of the assay method was performed according to the Japanese Pharmacopoeia and International Conference on Harmonization guidelines. As a result, we confirmed that capillary electrophoresis, including MEKC, is a powerful method for estimating the quality of natural medicines and traditional Chinese medicines consisting of various components.

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© 2003 by the PHARMACEUTICAL SOCIETY OF JAPAN
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