YAKUGAKU ZASSHI
Online ISSN : 1347-5231
Print ISSN : 0031-6903
ISSN-L : 0031-6903
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尿グルカリン酸測定法の改良とその応用
長谷川 浩平清水 久実代森本 茂文八野 芳已長谷川 健次庄野 嘉治堀内 哲也田伏 克惇桝井 友梨奈山口 敬子藤田 芳一
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2008 年 128 巻 1 号 p. 135-140

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  We propose an improved method to measure urinary D-glucaric acid (GA), which might be of value as an indirect index of the activity of cytochrome P450 (CYP). This method was about 20 times more sensitive than existing methods. Beer's law was obeyed in the concentration range 5.5-66 ng ml-1 for GA, with the effective molar absorptivity at 533 nm and the relative standard deviation being 9.1×105 dm3 mol-1 cm-1 and 0.69% (n=6), respectively. In addition, we introduced the correction value {GA/Cr ratio×10} of urinary GA by measuring urinary creatinine (Cr) at the same time. Based on the proposed method, the GA and Cr values in spot urines of healthy persons and cancer patients were subsequently measured and the correction values of both groups subjected to comparison. As a result, a statistically significant difference was recognized between the two groups.

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© 2008 by the PHARMACEUTICAL SOCIETY OF JAPAN
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