誌上シンポジウム
蛍光標識ペプチドツールで見る膜タンパク質の自己会合挙動
2019 年 139 巻 2 号 p. 273-276
詳細
2019 年 139 巻 2 号 p. 273-276
Detecting the behaviors of proteins in membranes is often challenging; we need to develop new methods to better understand the mechanisms involved. We have developed two types of peptide-based experimental systems that can detect the self-association of proteins in bilayer environments: 1) a single-pair fluorescence detection system for studying the self-association of transmembrane helices in model membranes; and 2) live-cell fluorescence labeling and analysis of the oligomeric state of membrane proteins using a coiled-coil labeling method. By using these methods, we show that membrane cholesterol significantly affects the self-association of transmembrane helices.
