高速液体クロマトグラフ法による医薬品の分析(第13報)葛根中のダイズインの定量
1980 年 100 巻 10 号 p. 1057-1060
詳細
1980 年 100 巻 10 号 p. 1057-1060
A method for the determination of daidzin in Puerariae Radix was established by using high-performance liquid chromatography. Daidzin in Puerariae Radix was separated on the 15cm column of Nucreosil ODS with an eluent of 18% acetonitrile, and detected at 254 nm by phothometric detector. Precision of the present method was about 1% and detection limit was about several ng (S/N ratio, about 3). Daidzin in Puerariae Radix was extracted with water and the extract was injected directly into the column. Sufficient separation of daidzin and other components in Puerariae Radix water extract was obtained. The content of daidzin was calculated from the calibration curve prepared previously by use of a standard. This method was considered to be useful for the evaluation of Puerariae Radix.
