ドテシル硫酸ナトリウム可溶化による組織チオバルビツール酸値の測定法

抄録

Determination procedures for thiobarbituric acid (TBA) reactive compounds in animal tissue homogenates were studied with sodium dodecylsulphate (SDS) as a tissue solubilizing agent. The treatment of tissue homogenate with SDS increased the TBA value, significantly, particularly, the addition of 2.5-fold SDS to protein concentration in homogenate showed maximum increase of the TBA value upon 30-min heating. The TBA increase, however, was not at a constant rate in all tissues. On the other hand, the effect of SDS was not as great in young rat tissue homogenate accelerated lipid peroxidation with the treatment of ascorbate-FeCl₂ or ascorbate-ADP mixture. These observations suggest that the differences in the efficiency of SDS on TBA depend on the physiological membrane conditions. These results suggest that the SDS tissue-solubilizing method is useful for the exact determination of TBA in animal tissues.