高速液体クロマトグラフィーによる血清中ブロムワレリル尿素の定量
1980 年 100 巻 6 号 p. 675-677
詳細
1980 年 100 巻 6 号 p. 675-677
A method for the determination of serum bromvalerylurea (BVU) was established, using high-speed liquid chromatography. BVU was extracted from serum with ethanol containing 3-nitro-p-anisidine as an internal standard, and chromatographed on a reversephase column (LiChrosorb RP-18, 5 μm) with a mobile phase of acetonitrile/water (1/3 (v/v)). This method facilifated BVU determination within 1.16 % error over the serum level range from 10 to 500 μg/ml, with the limit of detection being 10 μg/ml.
