1981 年 101 巻 2 号 p. 148-152
Assay of lysozyme chloride have been conducted by bacteriolytic method, by using Micrococus lysodeikticus as substrate. But the reproducibility of this method is invariably poor because of enzymatic processes in a heterogeneous system. We began to research the determination of lysozyme chloride by reversed phase high performance liquid chromatography. And we succeeded in retaining lysozyme chloride to reversed phase stationary, which is a basic condition for the determination by HPLC. We present here a HPLC system capable of retaining lysozyme chloride to reversed phase stationary by adding alkylsulfonic acid or perchloric acid as anion type counter ion to mobile phase. The effects of HPLC conditions on retention of lysozyme chloride were also examined. The good reproducibility could be suggested, when it was applied to the determination of lysozyme chloride.