1981 年 101 巻 3 号 p. 221-226
2-Thiobarbituric acid (TBA) values obtained by 1% H3PO4 method which is applicable to whole tissue homogenate (Anal. Biochem. 86, 271 (1978)) were further evaluated by using highly peroxidized CCl4-intoxicated rat liver homogenate along with the control one. Both homogenates were fractionated by three manners : Trichloroacetic acid treatment to yield supernatant and acid-insoluble precipitate ; chloroform-methanol extraction to obtain lipid, water soluble and residual fractions ; and 105000×g ultracentrifugation to make supernatant and pellet fractions. Of all fractions were measured conjugated diene and peroxide values besides TBA values and a good similarity of indices was obtained. However, the sum of the TBA value of each fraction was far smaller than that of original whole homogenate in CCl4-intoxicated rat, while the sum was rather bigger than original value in the case of normal rat, irrespective to the method of fractionation. Since the color development of TBA reaction is strongly affected by various cell components, it is likely that 1% H3PO4 method applied to whole homogenate does not give only the existing amount of TBA-reactive substances, but can represent the combined status of tissue peroxidation including several antioxidative and prooxidative factors located in other compartments from extracted lipid.