YAKUGAKU ZASSHI
Online ISSN : 1347-5231
Print ISSN : 0031-6903
ISSN-L : 0031-6903
蛋白の高速液体クロマトグラフィー(第2報)逆相高速液体クロマトグラフィーによる蛋白の分離
浅川 直樹津野 昌紀服部 禎一上山 政晤篠田 愛信三宅 康夫
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1981 年 101 巻 3 号 p. 279-282

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Recently several papers have shown that proteins can be separated by using gradient elution method in reversed phase partition high performance liquid chromatography (HPLC) system, but there has been no report on the use of isocratic elution method. The HPLC method, which has been developed in previous report, was examined whether it can be applied to achieve efficient separation and identification of proteins. Under isocratic elution system by use of Nucleosil CN as stationary phase and sodium octanesulfonate as ion-pair reagent in mobile phase, we studied simultaneous spearation of five kinds of proteins ; insulin, ribonuclease, myoglobin, lysozyme chloride and cytochrome C. Under optimal conditions all of the proteins tested could be satisfactorily chromatographed.

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