YAKUGAKU ZASSHI
Online ISSN : 1347-5231
Print ISSN : 0031-6903
ISSN-L : 0031-6903
ラット肝臓ミトコンドリヤのプロトヘムフェロリアーゼの精製
栄田 和子福永 浩一西依 健菊谷 元資
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1981 年 101 巻 3 号 p. 291-293

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From the rat liver mitochondria, protoheme ferro-lyase [E. C. 4.99.11], an enzyme localized on its inner membrane, was purified about 44-fold over the specific activity of the homogenate. The purification was carried out by (NH4)2SO4 fractionation, gel filtration on Sepharose-4B, column chromatographies on DEAE-cellulose and hydroxylapatite in the presence of detergents. From the results of DISC and SDS-polyacrylamide gel electrophoresis, this enzyme was estimated to be an oligomer enzyme with an approximate molecular weight of 380000 consisting of NA-H-1, an extrinsic protein on the inner membrane, and D-1 and D-2, intrinsic proteins linked by a hydrophobic bond to the inner membrane, and the approximate molecular weights of these subunit proteins were 120000, dimer of 74000 and 110000, respectively.

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© by the PHARMACEUTICAL SOCIETY OF JAPAN
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