Abstract
Glycyrrhizin in licorice root and its extract was determined by HLC with a reverse phase column. Glycyrrhizin was separated for about 10 min under the following operating conditions : column, μ-Bondapak C-18 ; mobile phase, 2% acetic acid-acetonitrile (20 : 11) ; internal standard, butyl p-hydroxybenzoate / temperature, room temperature. By comparing with thin layer chromatography, colorimetric method and gas chromatography, this HLC method was found to be excellent one in its simplicity, rapidity and accuracy. Commercially available white monoammonium glycyrrhizinate, monopotassium glycyrrhizinate, dipotassium glycyrrhizinate and glycyrrhizic acid were determined to be as low as 60-75% (as glycyrrhizic acid content) by using monoammonium glycyrrhizinate separated by HLC as a standard sample.
