マスフラグメントグラフィーによるN⁴-Behenoyl-1-β-D-arabinofuranosylcytosineの尿中代謝物Ara-U, Ara-Cの同時微量分析

抄録

A method for the determination of Ara-C and Ara-U, the major metabolites of N⁴-behenoyl-1-β-D-arabinofuranosylcytosine (BH-AC), was established by means of mass fragmentography. The acetylmethylated Ara-C and acetylated Ara-U were prepared and examined by gas chromatography-mass fragmentography (GC-MF). Each derivative was separated on 3% SE-30 column at 250°C. The contents of Ara-C and Ara-U were estimated from the calibration curve with papaverine as an internal standard. The urinary metabolites of BH-AC could be analyzed by this method without any extraction or separation procedure. This method is suitable for the selective analysis of Ara-C and Ara-U from the urinary metabolites of BH-AC.