細管式等速電気泳動法による医薬品の分析(第2報)製剤中のバルプロ酸ナトリウムの定量

抄録

A capillary-tube isotacophoretic procedure for the determination of valproic acid was established. Valproic acid was separated with the following mobile ions : leading electrolyte, 2.5 mM hydrochloric acid in 0.2% polyvinylalcohol adjusted to pH 5.50 by adding L-histidine ; terminating electrolyte, 2.5 mM 2-(N-morpholino) ethanesulfonic acid solution adjusted to pH 7.20 with tris (hydroxymethyl) aminomethane. Commercially available preparations of sodium valproate were determined without pretreatment such as the extraction with organic solvent and the derivation. The values of valproic acid determined by the method were in good agreement with those determined by gas-liquid chromatographic method. This method provides a simple and rapid technique for the determination of valproic acid.