3-Aminopropyltriethoxysilane処理薄層クロマトグラフィー用プレートの評価.ニンジンエキス分析への応用

抄録

Saponins from Panax ginseng were studied by thin layer chromatography (TLC) using an NH₂-chemically bonded stationary phase, prepared from pre-coated silica gel plate treated with benzene solution containing 3-aminopropyltriethoxysilane (3APTS). TLC plate treated with 3APTS was applied to the evaluation of the commercial "Ginseng Extract". After developement, 3APTS-treated TLC plates were dried, sprayed with sulfuric acid solution, and followed by heating. Each saponin spot on the chromatogram was measured with a TLC densitometer equipped with a dual-wave length TLC scanner at λ_s 525 nm (sample) and λ_R 760 nm (reference).