高速液体クロマトグラフィーによるアルキル化処理人免疫グロブリン製剤中の還元剤, アルキル化剤の定量
1982 年 102 巻 2 号 p. 215-220
詳細
1982 年 102 巻 2 号 p. 215-220
High performance liquid chromatography (HPLC) by use of a reverse phase column (TSK LS410K) was applied to determine the residual dithiothreitol, iodoacetamide and oxidized dithiothreitol in the alkylated human immunoglobulin (AHIG). After extraction by ethyl acetate, the above compounds were separated by HPLC with MeOH-H2O (60 : 40 v/v) as solvent. Determination was completed within 5 min with high reproducibility. Less than 0.5 ppm for each residual substance could be quantitated. The method was quite suitable for the routine analysis of the residual contaminants in AHIG.
