薄層スチッククロマトグラフィーの研究(第4報)競走馬尿中のドーピング薬物のスクリーニング法

抄録

The screenig tests of 34 doping drugs extracted with chloroform-n-butanol (95 : 5) from horse urine samples were examined on a thin-layer stick of silica gel containing mixed fluorescent substance and microcrystalline cellulose. After over-spotting of 1% ethanol solution of phenolphthalein as an internal standard substance, the stick was developed with chloroform : acetone : methanol (9 : 1 : 1). Thin-layer sticks developed with a solvent mixture were allowed to dry in air and then immersed for a short time in a color reagent. The analysis of horse urine containing polyethylene glycol (PEG) were examined with 2 kinds of developing solvents of n-hexane : acetone (1 : 1) and chloroform : methanol (3 : 1). Attempts for the detection of barbiturate were also made to determine whether dipping front of the chromatographic stick in the sample solution rather than spotting it with the sample solution might be satisfactorily efficient. The tests yielded gratifying results with respect to the efficiency of detection and with the simplicity of the procedure.