ガスクロマトグラフィーによる血漿中クロフェキサミドおよびその代謝産物の微量分析

抄録

A specific and sensitive gas chromatographic method was developed for the simultaneous determination of clofexamide, (2-(p-chlorophenoxy)-N-(2-diethylaminoethyl)-acetamide), and its two N-dealkylated metabolites in the plasma. The method involves a solvent extraction, trifluoroacetylation and analysis by gas chromatography on a 5% OV-17 column with flame thermionic detection. The N-(2-methylaminopropyl) analogue of clofexamide was used as an internal standard. The plasma concentration of each substance can be measured at the levels down to 4 ng/ml. The method was applied to determine the plasma levels of intact clofexamide and the metabolites after intravenous and oral administration to rabbits.