YAKUGAKU ZASSHI
Online ISSN : 1347-5231
Print ISSN : 0031-6903
ISSN-L : 0031-6903
Cyclic AMPのラジオイムノアッセイのB/F分離をセルハーベスターにより行う迅速簡便法
小山 岩雄桑江 豊保倉田 宗司
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1982 年 102 巻 7 号 p. 695-697

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The characteristics of the cell harvester (LM-101, Labo Mash, Labo Science Co.) were developed for separation of bound and free labeled antigens or haptens (B/F separation) in radioimmunoassay to determine cyclic AMP. Sensitivity and accuracy of the Labo Mash filtration method were similar to those of the Millipore filtration method or the double antibody method. The filtration method by use of the cell harvester is effective for B/F separation in radioimmunoassay, since this procedure is very quick and simple.

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© by the PHARMACEUTICAL SOCIETY OF JAPAN
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