高速液体クロマトグラフィーによる生体中のアデノシン-5'-トリホスフェートの定量
1983 年 103 巻 12 号 p. 1323-1326
詳細
1983 年 103 巻 12 号 p. 1323-1326
A high-performance liquid chromatographic method for the determination of adenosine-5'-triphosphate (ATP) in the biological materials, blood, heart, liver and kidney is described. The organs were frozen in liquid nitrogen immediately after the removal in situ with precooled tongs and homogenized with cold 10% trichloroacetic acid and 0.1 N HCl. The chromatographic analysis utilized an anion exchange (Polygosil N (CH3)2) column with a mobile phase of 0.35 M KH2PO4 buffer (pH3.35) and ultraviolet monitoring at 260 nm. This method was applied to the determination of ATP in the blood, heart, liver and kidney. The minimum detectable quantity of ATP was 10 pmol (about 55 ng) and the coefficient of variation was about 1%.
