Corynebacterium parvum, BCG, OK-432およびLactobacillus caseiのマウス肝薬物代謝酵素と肝マクロファージの活性酸素生成能におよぼす影響

抄録

Influence and mechanisms of four immunostimulants, Maycobacterium bovis (BCG), Streptococcus preparation (OK-432), Corynebacterium parvum (C. parvum), Lactobacillus casei YIT 9018 (LC 9018) and Lactobacillus fermentum YIT 0159 (LF), which had no immuno stimulating activity, on the activity of hepatic drug-metabolizing enzyme and on the activity of glucose-6-phosphate dehydrogenase (G6PD) and on the oxygen radical production by fixed macrophages in the liver of BALB/c mouse were studied. The levels of cytochrome P-450 and the activities of aminopyrine demethylase (APD), nicotinamide adenine dinucleotide phosphate-cytocrome c (NADPH-cytochrome c) reductase significantly decreased after the i. v. injection of BCG, LC 9018, OK-432, C. parvum and LF compared to the saline-injected control mice, but the level of cytochrome b₅ was not altered with these treatments. On the other hand, G6PD activity in the liver more remarkably increased with these immunostimulants than the saline i. v. injected mice, and oxygen radical production by fixed macrophages in the liver significantly increased after i. v. injection of LC 9018 and C. parvum. It was suggested that the ability to reduce the enzyme activities of APD and NADPH-cytochrome c reductase and the levels of cytochrome P-450 and cytochrome b₅ of the liver has no correlation with tumoricidal and bactericidal activity of these bacterial immunostimulants.