高速液体クロマトグラフィーによるヒト血漿中及び尿中Carteololの定量

抄録

A high performance liquid chromatographic procedure for the assay of carteolol in the human plasma and urine was investigated in the present study to simplify the procedure, to increase the assay sensitivity, and to established a more appropriate assay procedure for routine analysis than the fluorescent method. The chromatogram obtained under the following elution conditions : internal standard, 1-methyl-carteolol ; column, μ-Bondapak C₁₈ ; and mobile phase, a mixture of 30% acetonitrile, 0.02 M NH₄H₂PO₄ and 0.02 M (NH₄)₂HPO₄ ; in the human plasma and urine were satisfactory without interfering peak. The coefficients of correlation for the calibration curves in the plasma and urine were 0.9988 and 0.9998, respectively. The plasma and urinary concentrations of unchanged compound for the first 24 h after a single oral administration at 15 mg/body in healthy volunteers by this assay procedure were satisfactory. The correlation between the plasma concentration and urinary excretion rate of carteolol was good. Therefore, it was considered to be possible to evaluate the bioavailability of carteolol by using the urinary excretion rate.