1985 年 105 巻 6 号 p. 517-530
A new method for the selective modification of proteins which is capable to proceed under mild conditions, was developed by introducing a heterobifunctional reagent named N-(m-maleimide benzoyloxy) succinimide. Applications of the reagent in two new methods for enzyme labeling of antigens or antibodies, and for preparing drug immunogens were reviewed. Various enzyme immunoassay of drugs and hormons as well as antibodies were developed with the two immuno-reagents prepared by these methods. Also applications of these enzyme immunoassays for several studies are presented.