1985 年 105 巻 8 号 p. 770-774
The possibility of metabolism of dantrolene sodium with fresh stool was investigated because a low correlation was observed between dantrolene sodium dose and its serum concentrations. After combination of dantrolene with sterilized broth (pH 7.1) or 8% sterilized stool culture (pH 5.6) without further anaerobic precautions, dantrolene did not degrade in the incubation mixture. This indicate that dantrolene dose not react with the components of broth and stool cultures, and that it is stable at these pH values. On the other hand, after combination of dantrolene with 1% or 8% human faecal contents in a broth, the drug disappeared rapidly in the incubation mixture in proportion to stool concentration. The degradation of dantrolene was followed by a high performance liquid chromatography (HPLC) and the appearance of a metabolite was followed by HPLC and thin-layer chromatography (TLC). The metabolite was identified to be 7-aminodantrolene by HPLC and TLC.