1987 年 107 巻 2 号 p. 150-156
In new borns, measurement of 17α-hydroxyprogesterone (17-OHP) is used in the initial diagnosis of congenital adrenal hyperplasia (CAH) due to deficiency in steroid 21-hydroxylase. Therefore, we attempted to develop a highly sensitive enzyme immunoassay (EIA) for the determination of 17-OHP in dried blood samples. The specificity of various antibodies and sensitivity of various combinations of antibody and enzyme conjugate were studied. The most sensitive combination of antibody and enzyme conjugate was a bridge heterologous system by using anti-17-OHP-4-carboxyethylthioether-BSA antiserum and 17-OHP-4-carboxymethylthioether-horseradish peroxidase conjugate. The detection limit of this system was 0.25 pg/assay and its 50% displacement value was 2.6 pg/assay for 17-OHP. The application of this method for the determination of 17-OHP levels in dried blood disc of new born babies and the observation of 17-OHP levels of CAH babies were described.