クサウラベニタケの毒性成分の研究(第2報) : 溶血素の精製と溶血条件の検討
1988 年 108 巻 3 号 p. 221-225
詳細
1988 年 108 巻 3 号 p. 221-225
A fraction causing diarrhea to mice with a hemolytic activity was previously isolated from the aqueous extract of a poisonous mushroom Rhodophyllus rhodopolius. In order to clarify the relationship between the enterotoxicity and hemolytic activity, the hemolysin was purified. The hemolysin which was partially purified by DEAE-cellulose column chromatography, ammonium sulfate precipitation, and Sephacryl S-300 column chromatography, caused diarrhea and intestinal hemorrhage to suckling mice. The molecular weight of the hemolysin was estimated to be about 40000 by SDS-slab polyacrylamide gel electrophoresis and gel filtration. The hemolytic activity of the hemolysin was observed to be relatively heat-stable in the crude extract and dependent on the temperature and concentration of erythrocytes.
