ウロキナーゼの精製 (第1報) : アフィニティクロマトグラフィー担体の調製と特性
1988 年 108 巻 4 号 p. 331-338
詳細
1988 年 108 巻 4 号 p. 331-338
New affinity chromatographic solid supports were developed to obtain a high quality of urokinase (UK) preparation. The supports used 6-aminocaproic acid (6-ACA) as a spacer and p-aminobenzamidine (p-AB) as a ligand which were used by Holmberg et al. But the supports were prepared by verying the amount ratio of p-AB/6-ACA were covalently bound to Sepharose CL-6B. The optimum molar ratio of p-AB/6-ACA was found to be approximately 0.3 for the adsorption and desorption of UK. By the single purification step with this affinity chromatographic support, highly purified (116000-85000 IU/mg protein) and pyrogen free UK was successfully obtained from a crude preparation (3300 IU/mg protein) with a recovery of 86%. The activity ratio of high molecular weight UK (HUK)/low molecular weight UK (LUK) was found to be about 4.3.
