YAKUGAKU ZASSHI
Online ISSN : 1347-5231
Print ISSN : 0031-6903
ISSN-L : 0031-6903
二次蛍光検出高速液体クロマトグラフィーによるp-ベンゾキノン類の定量
高舘 明平賀 浩信藤野 博之合屋 周次郎
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1989 年 109 巻 2 号 p. 144-146

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A simple and sensitive high performance liquid chromatography (HPLC) with a fluorescence detection was developed for the determination of p-benzoquinones. The method was based on the reduction of p-benzoquinones to the corresponding fluorescent hydroquinones with sodium borohydride. The most sensitive and quantitative procedure was achieved when p-benzoquinones were reduced in methanol for 5 min and the fluorescence was monitored with the second-order emission. The detection limit of p-benzoquinone was 10 pg for an injection volume of 10 μl (S/N=2) and the coefficient of variation was found to be 2.60% (n=8) at 400 pg of p-benzoquinone.

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