1989 年 109 巻 2 号 p. 144-146
A simple and sensitive high performance liquid chromatography (HPLC) with a fluorescence detection was developed for the determination of p-benzoquinones. The method was based on the reduction of p-benzoquinones to the corresponding fluorescent hydroquinones with sodium borohydride. The most sensitive and quantitative procedure was achieved when p-benzoquinones were reduced in methanol for 5 min and the fluorescence was monitored with the second-order emission. The detection limit of p-benzoquinone was 10 pg for an injection volume of 10 μl (S/N=2) and the coefficient of variation was found to be 2.60% (n=8) at 400 pg of p-benzoquinone.