アルコール類の蛍光誘導体化試薬Pyrene-1-carbonyl Azideの合成及びその反応性

抄録

This paper describes the preparation of pyrene-1-carbonyl azide (PCA) which is applicable for the labeling of primary and secondary alcohols for high performance liquid chromatography (HPLC) with fluorescence detection and the optimum conditions for precolumn derivatization reaction. Alcohols were derivatized quantitatively into the corresponding fluorescent urethanes by treating with PCA at 60°C for 5 min. The derivatives were subjected to HPLC on a TSKgel ODS-120A (250×4.6 mm i.d., 5μm) column with acetonitrile-ethanol-water (70 : 29 : 1, v/v/v) as the mobile phase and monitored with an excitation wavelength of 342 nm and an emission wavelength of 389 nm. The detection limits for the alcohols were in the range 25-125 fmol per injection with a signal-to-noise ratio of 3.