アンモニア電極と精製Factor Cを用いたエンドトキシンの定量法
1992 年 112 巻 12 号 p. 940-943
詳細
1992 年 112 巻 12 号 p. 940-943
Factor C, an endotoxin-sensitive serine-protease zymogen, was prepared by cation-exchange chromatography using SP Toyopearl 650C. This preparation exhibited a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The endotoxine-dependent Factor C activity was determined from the amount of released ammonium ions by cleavage of N-tert-butoxy-carbonl Val-Pro-Arg-NH2 using an ammonia electrode. The sensitivity towards the endotoxin was approximately 5×10-8 g/ml.
