ヨウ素化合物の研究(第10報) : Glycyltyrosineのヨウ素化合物の単離精製法

抄録

The methods for the isolation and purification of iodoglycyltyrosines [glycyl-3-iodotyrosine (Gly-MIT) and glycyl-3, 5-diiodotyrosine (Gly-DIT)] from a reaction mixture were examined by the use of high-performance liquid chromatography (HPLC). Glycyltyrosine (Gly-Tyr) was iodinated with iodine monochloride (ICl)(the molar ratio of Gly-Tyr to ICl was 1 : 1.5) in 0.1 M NaOH. The synthesized Gly-MIT and Gly-DIT were separated on a μBondapak C₁₈ column employing stepwise gradient systems of a 0.1% trifluoroacetic acid/acetonitrile mixture and a water/acetonitrile mixture. Chemically pure Gly-MIT and Gly-DIT were obtained in 30.2% and 28.2% yields, respectively.